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megfp c1 construct  (Addgene inc)


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    Structured Review

    Addgene inc megfp c1 construct
    Megfp C1 Construct, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 82 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/megfp+c1/mEGFP-C1+(Plasmid+%2354759)/10__1038_slash_s41467___026___78058___y-607-22-24
    Average 94 stars, based on 82 article reviews
    megfp c1 construct - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Clone Assay:

    Article Title: G3BP isoforms differentially affect stress granule assembly and gene expression during cellular stress
    Article Snippet: Stress granules (SGs) are macromolecular assemblies that form under cellular stress.. Formation of these membrane-less organelles is driven by the condensation of RNA and RNA-binding proteins such as G3BPs.. G3BPs form SGs following stress-induced translational arrest.

    Article Title: BLTP1-dependent phospholipid efflux prevents mitochondrial lipid overload and apoptosis at FKBP8-tethered ER-mitochondrial contact
    Article Snippet: .. 3xFlag-6xHis-BLTP1(residues 68-665) was cloned into 3xFlag-C1 vector between HindIII and BamHI. mCherry-C1 and 3xFlag-C1 were constructed by replacing the GFP tag in mEGFP-C1 (Addgene 54579) vector with mCherry or 3xFlag via AgeI and NotI.14xHis-NEDD8-FKBP8-ΔTM was cloned into 14xHis-NEDD8 vector between BamHI and HindIII. .. To construct BLTP1 KO cell lines, two gRNAs (5’-AGAATGGGAAAACAAATCAG-3’ and 5’-CTTCCCCAACCGATTGATTG-3’) were used.Complementary gRNAs were annealed and subcloned into the pSpCas9(BB)-2A-GFP (pX-458) vector (#48138; Addgene) between BbsI endonuclease restriction sites.

    Plasmid Preparation:

    Article Title: G3BP isoforms differentially affect stress granule assembly and gene expression during cellular stress
    Article Snippet: Stress granules (SGs) are macromolecular assemblies that form under cellular stress.. Formation of these membrane-less organelles is driven by the condensation of RNA and RNA-binding proteins such as G3BPs.. G3BPs form SGs following stress-induced translational arrest.

    Article Title: ORP9-PH domain-based fluorescent reporters for visualizing phosphatidylinositol 4-phosphate dynamics in living cells
    Article Snippet: .. ; pAcGFP1-Golgi from Clontech; mCherry-P4M-SidM (Addgene plasmid #51471), iRFP-FRB-Rab5 (Addgene plasmid #51612), and iRFP-FRB-Rab7 (Addgene plasmid #51613) from Dr Tamas Balla (National Institute of Health); pLAMP1-miRFP703 (Addgene plasmid #79998) and pmiRFP670-N1 (Addgene plasmid #79987) from Dr Vladislav Verkhusha (Albert Einstein College of Medicine); GFP-C1-PLCdelta-PH (Addgene plasmid #21179) from Dr Tobias Meyer (Weill Cornell Medical College of Cornell University); mEGFP-C1 (Addgene plasmid #54759) and mTagBFP2-Lifeact-7 (Addgene plasmid #54602) from Dr Michael W. Davidson (Florida State University); and pC 4 EN-F1 (encoding FKBP12) and pC 4 -R H E [encoding FRB(T2098L)] from ARIAD Pharmaceuticals. ..

    Article Title: Phylogenomic, structural, and cell biological analyses reveal that Stenotrophomonas maltophilia replicates in acidified Rab7A-positive vacuoles of Acanthamoeba castellanii
    Article Snippet: Neff:mEGFP-rab7a , Acanthamoeba castellanii Neff derivative constitutively expressing a mEGFP-Rab7A fusion protein after transfection with pAcaGAPDH_mEGFP-rab7a , This work. .. mEGFP-C1 , Mammalian expression vector, npt II gene expressed from SV40 and AmpR promoters; mEGFP under control of the CMV promoter and enhancer; 4,731 bp. , AddGene #54759. .. mEGFP-N1 , Mammalian expression vector, npt II gene expressed from SV40 and AmpR promoters; mEGFP under control of the CMV promoter and enhancer; 4,733 bp. , AddGene #54767.

    Expressing:

    Article Title: Phylogenomic, structural, and cell biological analyses reveal that Stenotrophomonas maltophilia replicates in acidified Rab7A-positive vacuoles of Acanthamoeba castellanii
    Article Snippet: pAcaPrTPB_bkbn_mCherry2-N1 , mCherry2-N1 derivative with expression of the npt II gene under control of the A. castellanii TBP promoter region (193 bp); 4,573 bp. , This work. .. pAcaGAPDH_mEGFP-C1 , A. castellanii backbone expression vector derived from mEGFP-C1 by putting the npt II gene under control of the A. castellanii TBP promoter region and the mEGFP fusion tag expressed from the amoebal GAPDH promoter region (751 bp); 4,733 bp. , This work AddGene #208175. .. pAcaGAPDH_mEGFP-N1 , A. castellanii backbone expression vector derived from mEGFP-N1 by putting the npt II gene under control of the A. castellanii TBP promoter region and the mEGFP fusion tag expressed from the amoebal GAPDH promoter region (751 bp); 4,735 bp. , This work AddGene #208182.

    Article Title: Phylogenomic, structural, and cell biological analyses reveal that Stenotrophomonas maltophilia replicates in acidified Rab7A-positive vacuoles of Acanthamoeba castellanii
    Article Snippet: Neff:mEGFP-rab7a , Acanthamoeba castellanii Neff derivative constitutively expressing a mEGFP-Rab7A fusion protein after transfection with pAcaGAPDH_mEGFP-rab7a , This work. .. mEGFP-C1 , Mammalian expression vector, npt II gene expressed from SV40 and AmpR promoters; mEGFP under control of the CMV promoter and enhancer; 4,731 bp. , AddGene #54759. .. mEGFP-N1 , Mammalian expression vector, npt II gene expressed from SV40 and AmpR promoters; mEGFP under control of the CMV promoter and enhancer; 4,733 bp. , AddGene #54767.

    Derivative Assay:

    Article Title: Phylogenomic, structural, and cell biological analyses reveal that Stenotrophomonas maltophilia replicates in acidified Rab7A-positive vacuoles of Acanthamoeba castellanii
    Article Snippet: pAcaPrTPB_bkbn_mCherry2-N1 , mCherry2-N1 derivative with expression of the npt II gene under control of the A. castellanii TBP promoter region (193 bp); 4,573 bp. , This work. .. pAcaGAPDH_mEGFP-C1 , A. castellanii backbone expression vector derived from mEGFP-C1 by putting the npt II gene under control of the A. castellanii TBP promoter region and the mEGFP fusion tag expressed from the amoebal GAPDH promoter region (751 bp); 4,733 bp. , This work AddGene #208175. .. pAcaGAPDH_mEGFP-N1 , A. castellanii backbone expression vector derived from mEGFP-N1 by putting the npt II gene under control of the A. castellanii TBP promoter region and the mEGFP fusion tag expressed from the amoebal GAPDH promoter region (751 bp); 4,735 bp. , This work AddGene #208182.

    Control:

    Article Title: Phylogenomic, structural, and cell biological analyses reveal that Stenotrophomonas maltophilia replicates in acidified Rab7A-positive vacuoles of Acanthamoeba castellanii
    Article Snippet: pAcaPrTPB_bkbn_mCherry2-N1 , mCherry2-N1 derivative with expression of the npt II gene under control of the A. castellanii TBP promoter region (193 bp); 4,573 bp. , This work. .. pAcaGAPDH_mEGFP-C1 , A. castellanii backbone expression vector derived from mEGFP-C1 by putting the npt II gene under control of the A. castellanii TBP promoter region and the mEGFP fusion tag expressed from the amoebal GAPDH promoter region (751 bp); 4,733 bp. , This work AddGene #208175. .. pAcaGAPDH_mEGFP-N1 , A. castellanii backbone expression vector derived from mEGFP-N1 by putting the npt II gene under control of the A. castellanii TBP promoter region and the mEGFP fusion tag expressed from the amoebal GAPDH promoter region (751 bp); 4,735 bp. , This work AddGene #208182.

    Article Title: Phylogenomic, structural, and cell biological analyses reveal that Stenotrophomonas maltophilia replicates in acidified Rab7A-positive vacuoles of Acanthamoeba castellanii
    Article Snippet: Neff:mEGFP-rab7a , Acanthamoeba castellanii Neff derivative constitutively expressing a mEGFP-Rab7A fusion protein after transfection with pAcaGAPDH_mEGFP-rab7a , This work. .. mEGFP-C1 , Mammalian expression vector, npt II gene expressed from SV40 and AmpR promoters; mEGFP under control of the CMV promoter and enhancer; 4,731 bp. , AddGene #54759. .. mEGFP-N1 , Mammalian expression vector, npt II gene expressed from SV40 and AmpR promoters; mEGFP under control of the CMV promoter and enhancer; 4,733 bp. , AddGene #54767.

    Amplification:

    Article Title: Hetero-oligomerization of TDP-43 carboxy-terminal fragments with cellular proteins contributes to proteotoxicity
    Article Snippet: .. DNA fragments for C-terminally CRY2olig-tagged meGFP were amplified by PCR from meGFP-C1 using the following primers: 5′- GCA GCTAGC CACCATGGTGAGCAAGGGCGAGGAG-3′ (forward side) and 5′- CGT CTCGAG CTTGTACAGCTCGTCCATGCCG-3′ (reverse side); then inserted into pCRY2olig-mCherry (Addgene) at the NheI and XhoI sites (pGFP-CRY-R). .. Murine neuroblastoma Neuro-2a cells (#CCL-131; ATCC, Manassas, VA, USA) and maintained in DMEM (D5796; Sigma-Aldrich, St. Louis, MO, USA) supplemented with 10% FBS (12676029, Thermo Fisher), 100 U/mL penicillin G (Sigma-Aldrich), and 0.1 mg/ml streptomycin (Sigma-Aldrich) .

    Polymerase Chain Reaction:

    Article Title: Hetero-oligomerization of TDP-43 carboxy-terminal fragments with cellular proteins contributes to proteotoxicity
    Article Snippet: .. DNA fragments for C-terminally CRY2olig-tagged meGFP were amplified by PCR from meGFP-C1 using the following primers: 5′- GCA GCTAGC CACCATGGTGAGCAAGGGCGAGGAG-3′ (forward side) and 5′- CGT CTCGAG CTTGTACAGCTCGTCCATGCCG-3′ (reverse side); then inserted into pCRY2olig-mCherry (Addgene) at the NheI and XhoI sites (pGFP-CRY-R). .. Murine neuroblastoma Neuro-2a cells (#CCL-131; ATCC, Manassas, VA, USA) and maintained in DMEM (D5796; Sigma-Aldrich, St. Louis, MO, USA) supplemented with 10% FBS (12676029, Thermo Fisher), 100 U/mL penicillin G (Sigma-Aldrich), and 0.1 mg/ml streptomycin (Sigma-Aldrich) .

    Construct:

    Article Title: BLTP1-dependent phospholipid efflux prevents mitochondrial lipid overload and apoptosis at FKBP8-tethered ER-mitochondrial contact
    Article Snippet: .. 3xFlag-6xHis-BLTP1(residues 68-665) was cloned into 3xFlag-C1 vector between HindIII and BamHI. mCherry-C1 and 3xFlag-C1 were constructed by replacing the GFP tag in mEGFP-C1 (Addgene 54579) vector with mCherry or 3xFlag via AgeI and NotI.14xHis-NEDD8-FKBP8-ΔTM was cloned into 14xHis-NEDD8 vector between BamHI and HindIII. .. To construct BLTP1 KO cell lines, two gRNAs (5’-AGAATGGGAAAACAAATCAG-3’ and 5’-CTTCCCCAACCGATTGATTG-3’) were used.Complementary gRNAs were annealed and subcloned into the pSpCas9(BB)-2A-GFP (pX-458) vector (#48138; Addgene) between BbsI endonuclease restriction sites.



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